Archive for May, 2012

Blog Entry on E. Coli Lab

Posted: May 7, 2012 in Uncategorized

DNA undergoes a process in which it gets translated into RNA and that gets translated into proteins. The process starts by DNA helicase attaches to the DNA and separates part of the DNA into 2 strands.One of these strands gets translated into a RNA strand. First RNA polymerase attaches to the strand and moves and translates the DNA into RNA. The RNA then detaches from the DNA. It gets shortened by getting all the exons. The shortened RNA strand then attaches itself to a Ribsosome. There it gets matched up with different tRNA which carry different amino acids with attach together to form a polypeptide chain or protein.

These photos show our different batches with or without the pGLO Plasmid.

Our data showed that the -pGLO LB had a lot of growth because we had 1 colony.

The -pGLO LB/AMP had no growth because the ampicillan would kill all the bacteria

The +pGLO LB/AMP had some growth because the pGLO would integrate itself into the bacteria and give it the bacterial resistance.

he +pGLO LB/AMP/ARA had a fair amount of growth along with the ability to glow in UV light, because the pGLO would integrate itself into the bacteria and give it these abilities